Effect of Extenders Including High Concentrations Dimethyl Sulfoxide (DMSO) on Post-Thaw Rabbit Sperm Parameters
نویسندگان
چکیده
Cryoprotectants have critical roles to prevent cell damages during cryopreservation. However, the adjustment of cryoprotectant concentration is also very crucial protect cells from toxicity. The present study was designed investigate effect extenders including high dimethyl sulfoxide (DMSO) on post-thaw rabbit sperm quality. Pooled samples (n=7) were diluted and cryopreserved in 250 mmol/L Tris, 88 citric acid, 47 glucose, 1% sucrose different concentrations DMSO. presence DMSO (12 14%) extender decreased total progressive motility (P < 0.01). 8 10 percent supplementations increased live rates Live intact acrosome or membrane detected higher groups 0.05). When similar all groups, rate group compared 12 In conclusion, when 14 additions adversely affected parameters, provided highest
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ژورنال
عنوان ژورنال: Journal of research in veterinary medicine
سال: 2022
ISSN: ['2667-6745']
DOI: https://doi.org/10.30782/jrvm.1125759