Effect of donor human milk on host-gut microbiota and metabolic interactions in preterm infants

Background & aimsHuman milk is the gold standard for infant nutrition. Preterm infants whose mothers are unable to provide sufficient own mother's (OMM), receive pasteurized donor human (DHM). We studied metabolic signatures of OMM and DHM their effect on interplay developing microbiota infant's metabolism.MethodsMetabolic fingerprinting as well urine was performed using liquid chromatography–mass spectrometry stool analyzed by 16S rRNA sequencing.ResultsSignificant differences in galactose starch sucrose metabolism pathways when comparing DHM, alterations steroid hormone synthesis pyrimidine were observed depending type feeding. Differences gut-microbiota composition also identified.ConclusionThe differs from feeding has a significant impact phenotype preterm infants. Our data help understand origin changes generating new hypothesis: i) hormones present HM have influence activity biosynthesis pathway infants; ii) modulated gut-microbiota. Short- long-term implications need be assessed further studies. Human metabolism. Metabolic sequencing. Significant identified. The