Effect of Different Antioxidant Sources Added to Buffalo Semen Extender During Cryopreservation on Freezability and Fertility of Buffalo Spermatozoa

نویسندگان

چکیده

| There are several types of antioxidants which can be use in buffalo semen preservation. The current study was performed to the influence viz., Coenzyme Q10 (CoQ10), L-Carnitine (LC), or N-Acetyl-Cysteine (NAC) added extender on freezability and fertility spermatozoa. Semen Egyptian bulls (n=5) were pooled extended with tris (1:10) without addition antioxidant (E1), i.e., 0.03 mM CoQ10 (E2), 3.0 LC (E3), 1.0 NAC (E4). Then diluted frozen at -196oC liquid nitrogen. Results showed that progressive motility, livability, membrane integrity, head-head agglutination percentages increased, while sperm abnormality acrosomal damage decreased by all supplements (P<0.05), being best for E2. Pregnancy rate higher (P<0.05) cows inseminated E2 E4 (81.8% each) versus 72.7 63.6% E3 E1, respectively. In conclusion, tris-egg yolk supplemented improve characteristics cryopreserved increase pregnancy cows.

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ژورنال

عنوان ژورنال: Journal of animal health and production

سال: 2021

ISSN: ['2308-2801']

DOI: https://doi.org/10.17582/journal.jahp/2021/9.3.222.228