Effect of Chicken Serum and Horse Serum on Proliferation and Differentiation of Chicken Muscle Satellite Cells

For efficient production of cultured meat, chicken breast muscle cells and leg were proliferated differentiated using medium with various serums, proliferation differentiation ability analyzed. Chicken serum-free (NC), 20% FBS (C), 10% serum (CS) 5% horse (HS) (T1), CS (T2), HS (T3). Using the immunofluorescence staining, Pax7 nuclei stained in to confirm that they satellite cells. As a result cell counting, both T1 most. subcultured from passage 1 6 medium. In cells, viability number live decreased increasing passage, but had higher than all passages. Cells containing for each passage. It was confirmed more maintained as increased Therefore, it is considered results will become basic data development meat.