Effect of Centella asiatica Extract on Anti-obesity Suppression via Inhibition of Adipogenesis-related Gene Expression in Preadipocyte

The present study was conducted to investigate the anti-oxidant and anti-obesity activities of Centella asiatica hot-water extract (CHE). Total polyphenol content radical scavenging activity were evaluated for CHE, 14.4 mg GAE/g DM 82.6% measured, respectively. Lipase inhibition, an marker, measured as 68.1%, confirming that triglyceride hydrolysis significantly inhibited by CHE. To mechanism, mRNA expressions peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (CEBP-α), fatty acid synthase (FAS), stearoyl-CoA desaturase1 (SCD1) in 3T3-L1 evaluated. level expression suppressed 1.52, 1.81, 1.13, 1.18 times, respectively, compared control group, CHE had effect inhibiting adipocytes development lipid accumulation. These results indicate can be used a raw material functional foods pharmaceuticals with potential reducing lipase preadipocyte differentiation.