Effect of anticoagulants on the chromogenic Limulus lysate assay for endotoxin.
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چکیده
منابع مشابه
Single-step, chromogenic Limulus amebocyte lysate assay for endotoxin.
A new reagent for the chromogenic Limulus amebocyte lysate (LAL) assay is described. LAL was formulated for optimal performance in either an endpoint procedure or a kinetic procedure with the chromogenic substrate, buffer, and LAL components colyophilized as a single reagent. The kinetic chromogenic method required an incubating microplate reader coupled to a computer for collection and analysi...
متن کاملModified micromethod of the Limulus amoebocyte lysate assay for endotoxin.
A Limulus amoebocyte lysate microtechnique performed in petrolatum wells on a microscope slide is described. Injection of a dye solution in ethanol directly into the wells leads to an unambiguous interpretation of the results. Twelve samples can be tested on a single slide, and compact storing of the samples is possible.
متن کاملEndotoxin Detection in Pharmaceuticals and Medical Devices with Kinetic-QCL, a Kinetic-Quantitative Chromogenic Limulus Amebocyte Lysate Assay.
The observation that endotoxin caused gelation in extracts of Limulus amebocytes has been expanded to the development of an in vitro kinetic, quantitative chromogenic LAL assay (Kinetic-QCL) for the detection of endotoxin in aqueous fluids. Within the last 15 years, the use of Limulus amebocyte lysate to detect and control the presence of pyrogenic substances in pharmaceuticals and medical devi...
متن کاملChromogenic Limulus amoebocyte lysate assay for rapid detection of gram-negative bacteriuria.
A chromogenic Limulus amoebocyte lysate assay was evaluated as a rapid screening test for the detection of clinically significant gram-negative bacteriuria. The development of a distinctive yellow color after the addition of chromogenic substrate to the Limulus amoebocyte lysate-urine reaction mixture was used to measure greater than or equal to 10(5) gram-negative bacteria per ml. A total of 3...
متن کاملAdaptation of a microdilution procedure to the Limulus lysate assay for endotoxin.
Endpoint determinations of endotoxin by the Limulus amoebocyte lysate assay have been facilitated by use of a microdilution procedure.
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1985
ISSN: 0021-9746
DOI: 10.1136/jcp.38.4.430