Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae)

Organophosphate pesticides, especially chlorpyrifos, are one of the most widely used insecticides in agriculture, but their toxicity and potential sensitivity effects on Anura, Fejervarya limnocharis still unknown. The purpose this investigation is to study F. (Anura: Dicroglossidae) tadpole lethal (survivability) sublethal (morphological alterations swimming activity) chlorpyrifos Dursban 200EC commercial formula under acute exposure. An test was carried out ten tadpoles (Gosner 25) each repetition. sample obtained from artificial reproduction by injecting Trial Batch 2000 IU hCG Kings Lab. testing consisted three replicates with a concentration 0; 0.4; 0.8; 1; 2; 4; 8 µg/L. Physico-chemical parameters, mortality, morphological, were observed at 24th, 48th, 72nd, 96th hours. LC50 for 2.86 percentage survivability decreased after exposure above 1µg/L, while morphological 2 µg/L 4 48th hours exposure, have occurred 24th Morphological including asymmetrical body shape, edema, abnormal tail shape. Based value, has high-level tadpoles.Keywords: Acute, Chlorpyrifos, 200 EC, limnocharis, Tadpoles