EB1 regulates microtubule dynamics and tubulin sheet closure in vitro
نویسندگان
چکیده
منابع مشابه
EB 1 promotes microtubule dynamics and regulates tubulin sheet closure in vitro
1 EB1 promotes microtubule dynamics and regulates tubulin sheet closure in vitro. End binding 1 (EB1) is a plus-end-tracking protein (+TIP) that localizes to microtubule plus ends where it modulates their dynamics and interactions with intracellular organelles 1, 2. Although the regulating activity of EB1 on microtubule dynamics has been studied in cells 3-6 and purified systems 7-13 , the mole...
متن کاملγ-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning.
γ-Tubulin is critical for microtubule (MT) assembly and organization. In metazoa, this protein acts in multiprotein complexes called γ-Tubulin Ring Complexes (γ-TuRCs). While the subunits that constitute γ-Tubulin Small Complexes (γ-TuSCs), the core of the MT nucleation machinery, are essential, mutation of γ-TuRC-specific proteins in Drosophila causes sterility and morphological abnormalities ...
متن کاملTTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation.
Microtubules (MTs) play critical roles in various cellular events, including cell migration. End-binding proteins (EBs) accumulate at the ends of growing MTs and regulate MT end dynamics by recruiting other plus end-tracking proteins (+TIPs). However, how EBs contribute to MT dynamics through +TIPs remains elusive. We focused on tau-tubulin kinase 2 (TTBK2) as an EB1/3-binding kinase and confir...
متن کاملMAP1B regulates microtubule dynamics by sequestering EB1/3 in the cytosol of developing neuronal cells.
MAP1B, a structural microtubule (MT)-associated protein highly expressed in developing neurons, plays a key role in neurite and axon extension. However, not all molecular mechanisms by which MAP1B controls MT dynamics during these processes have been revealed. Here, we show that MAP1B interacts directly with EB1 and EB3 (EBs), two core 'microtubule plus-end tracking proteins' (+TIPs), and seque...
متن کاملTubulin Post-Translational Modifications and Microtubule Dynamics
Microtubules are hollow tube-like polymeric structures composed of α,β-tubulin heterodimers. They play an important role in numerous cellular processes, including intracellular transport, cell motility and segregation of the chromosomes during cell division. Moreover, microtubule doublets or triplets form a scaffold of a cilium, centriole and basal body, respectively. To perform such diverse fu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Cell Biology
سال: 2008
ISSN: 1465-7392,1476-4679
DOI: 10.1038/ncb1703