Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters
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چکیده
منابع مشابه
Design of Superparamagnetic Iron Oxide Nanoparticle for Purification of Histidine- Tagged Recombinant Proteins
We report a novel purification system for 6 His-tagged proteins by magnetic affinity separation. We have developed superparamagnetic Fe3O4@SiO core-shell particles with immobilized surface iminodiacetate groups to chelate with Ni. This Ni magnetic silica nanoparticle has been shown as an efficient carrier, binder and anchor to obtain his-tagged protein directly from total cell lysate. The struc...
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The decoration of iron oxide nanoparticles with Ni2+ ions provided the superparamagnetic nanoparticles with a binding site for His-tagged proteins, allowing their selective binding and convenient separation from a multi-component solution with an appropriately applied magnetic field.
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ژورنال
عنوان ژورنال: Journal of Materials Chemistry
سال: 2011
ISSN: 0959-9428,1364-5501
DOI: 10.1039/c0jm04089d