Early keratocyte apoptosis after epithelial scrape injury in the human cornea
نویسندگان
چکیده
منابع مشابه
Keratocyte density in the human cornea after photorefractive keratectomy.
OBJECTIVE To perform a quantitative analysis of keratocyte density in human corneas after photorefractive keratectomy (PRK). METHODS In a prospective comparative trial, 24 eyes of 14 patients received PRK to correct refractive errors of between -1.25 diopters (D) and -5.75 D. Corneas were examined by using confocal microscopy before and 1 day, 5 days, and 1, 3, 6, 12, 24, and 36 months after ...
متن کاملEffects of mitomycin C on infiltration of polymorphonuclear leukocytes after epithelial scrape injury in the mouse cornea.
PURPOSE To determine whether mitomycin C (MMC) alters appearance and disappearance of polymorphonuclear leucocytes (PMN) in the cornea stroma, using an epithelial scrape injury in eye mouse model. METHODS Twenty-mice underwent mechanical epithelium debridement in the central cornea using 20% ethanol. After the scrape, the right eye received 0.02% MMC for one minute, while the left eye receive...
متن کاملRole of tears in keratocyte loss after epithelial removal in mouse cornea.
PURPOSE To study the role of tears in the death of keratocytes after epithelium removal in the mouse cornea. METHODS In anesthetized mice, an approximately 1-mm circle of epithelium was removed from the center of the cornea, exposing the underlying stroma. In one group of animals, access of tears to the bare stroma was allowed-in vivo, by closing the eyelids, or ex vivo, by dropping tears fro...
متن کاملKeratocyte apoptosis after corneal surgery.
PURPOSE Programmed cell death (apoptosis) is the controlled death of cells that occurs with minimal collateral damage to surrounding cells or tissue during development, homeostasis, and wound healing. The authors hypothesize the keratocyte apoptosis is an initiating factor in the wound-healing response after refractive surgical procedures. To evaluate the effects of different corneal manipulati...
متن کاملKeratocyte networks visualised in the living cornea using vital dyes.
Fluorescent viability probes have been used to visualise and investigate the viability, morphology and organisation of the keratocyte within the stroma of the intact living cornea. The live cell probe, calcien-AM, in combination with a dead cell probe, ethidium homodimer (Live/Dead Assay, Molecular Probes, U.S.A.) proved superior to earlier generation vital dyes such as fluorescein diacetate or...
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ژورنال
عنوان ژورنال: Experimental Eye Research
سال: 2009
ISSN: 0014-4835
DOI: 10.1016/j.exer.2009.06.003