Early events in the pathogenicity of Pseudomonas syringae on Nicotiana benthamiana
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Calcium efflux as a component of the hypersensitive response of Nicotiana benthamiana to Pseudomonas syringae.
Using a model plant Nicotiana benthamiana, we have demonstrated that initial calcium uptake in response to the HR (hypersensitive response)-causing pathogen Pseudomonas syringae pv syringae 61 is followed by net calcium efflux initiated at about 12 h after the bacterial challenge and sustained for at least 48 h. Our data suggest that calcium not only acts as an important second messenger in the...
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To explore possible sources of transgenic resistance to the rhizomania-causing Beet necrotic yellow vein virus (BNYVV), Nicotiana benthamiana plants were constructed to express the harpin of Pseudomonas syringae pv. phaseolicola (HrpZ(Psph)). The HrpZ protein was expressed as an N-terminal fusion to the PR1 signal peptide (SP/HrpZ) to direct harpin accumulation to the plant apoplast. Transgene ...
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Retraction: 'Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana'.
Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of ...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2007
ISSN: 0960-7412
DOI: 10.1111/j.1365-313x.2006.02981.x