EARLY EVENTS IN LYMPHOCYTE TRANSFORMATION BY PHYTOHEMAGGLUTININ

Early Events in Lymphocyte Transformation by Phytohemagglutinin

The DNA-dependent RNA polymerase activities of isolated nuclei from lymphocytes were examined after stimulation with phytohemagglutinin (PHA). The nuclear fraction was prepared with Mg(++) or Mn(++) to distinguish between polymerase I (nucleolar) and polymerase II (nucleoplasmic). Distinction between polymerases II and III was obtained by the addition of alpha-amanitin to the reaction mixture. ...

Effect of DDS on phytohemagglutinin-induced lymphocyte transformation.

The influence of DDS on PHA-induced lymphocyte transformation was investigated in leukocyte cultures from two samples of healthy Caucasoid individuals. In one sample the sulfone-treated cultures differed from the controls in that they contained 0.4 mug/ml of other sample, the treated cultures contained DDS in concentrations of 4 mug/ml, 8 mug/ml and 16 mug/ml. The frequency of lymphocyte transf...

Inhibitory effect of L-asparaginase in lymphocyte transformation induced by phytohemagglutinin.

The effect of Escherichia coli L-asparaginase on phytohemagglutinin (PHA)-induced transformation of human lymphocytes was studied. Transformation was remarkably suppressed by the enzyme when it was added to the cul ture not later than 24 hr after its initiation. The effective concentration was more than 0.2 i.u./tube. Guinea pig serum and L-0-aspartohydroxamic acid, an L-asparagine analog, have...

Quantitative dinitrochlorobenzene (DNCB) responsivity and phytohemagglutinin (PHA) induced lymphocyte transformation in patients with lepromatous leprosy.

Lymphocyte stimulation by phytohemagglutinin and tumor cells of malignant effusions.