Dysfunctional Factor VII Variant (FVII Tondabayashi) with R79Q: Determination of Mutated Site with Monoclonal Anti-Human Factor VII Antibody (B101/B1)

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Dysfunctional Factor VII Variant (FVII Tondabayashi) with R79Q: Determination of Mutated Site with Mono- clonal Anti-Human Factor VII Antibody

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Monoclonal Anti - human Factor VII Antibodies

Several murine monoclonal anti-human Factor VII antibodies were produced using hybridoma technology. Two noncompetitive monoclonal antibodies were used to examine by Western blotting the Factor VII cross-reactive material (CRM) in normal human plasma and three commercially available congenitally Factor VIIdeficient plasmas, and to construct a facile "sandwich" immunoassay for plasma Factor VII....

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Factor VII gene intronic mutation in a lethal Factor VII deficiency: effects on splice-site selection Short title Splicing at cryptic site in lethal FVII deficiency Authors

In a patient with lethal Factor (F) VII deficiency, two homozygous nucleotide substitutions were identified in the FVII gene: a IVS7+2T>G transversion involving the IVS7 donor splice site, followed by a mutation at nucleotide 10588 that would result in a missense variation (R224Q). The mutated splice site, located within the first repeat of a minisatellite, is followed by a variable number of p...

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Preparation of factor VII concentrate using CNBr-activated

  Background: Factor VII concentrates are used in patients with congenital or acquired factor VII deficiency or treatment of hemophilia patients with inhibitors. In this research, immunoaffinity chromatography was used to purify factor VII from prothrombin complex (Prothrombin-Proconvertin-Stuart Factor-Antihemophilic Factor B or PPSB) which contains coagulation factors II, VII, IX and X. The a...

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Factor VII verona coagulation disorder: double heterozygosis with an abnormal factor VII and heterozygous factor VII deficiency.

A family with a peculiar defect of factor VII is described. The propositi, a brother and sister, were born of a nonconsanguineous marriage and presented a mild to moderate bleeding tendency since childhood (epistaxis, excessive bleeding after tooth extraction). The coagulation pattern was characterized by a mildly prolonged prothrombin time, normal partial thromboplastin time, and normal Stypve...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1998

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/44.9.1993