Dynamic Halbach array magnet integrated microfluidic system for the continuous-flow separation of rare tumor cells
نویسندگان
چکیده
منابع مشابه
Combined microfluidic-micromagnetic separation of living cells in continuous flow.
This paper describes a miniaturized, integrated, microfluidic device that can pull molecules and living cells bound to magnetic particles from one laminar flow path to another by applying a local magnetic field gradient, and thus selectively remove them from flowing biological fluids without any wash steps. To accomplish this, a microfabricated high-gradient magnetic field concentrator (HGMC) w...
متن کاملContinuous Flow Deformability-Based Separation of Circulating Tumor Cells Using Microfluidic Ratchets.
Circulating tumor cells (CTCs) offer tremendous potential for the detection and characterization of cancer. A key challenge for their isolation and subsequent analysis is the extreme rarity of these cells in circulation. Here, a novel label-free method is described to enrich viable CTCs directly from whole blood based on their distinct deformability relative to hematological cells. This mechani...
متن کاملAn Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells
Genetic and transcriptional profiling, as well as surface marker identification of single circulating tumor cells (CTCs) have been demonstrated. However, quantitatively profiling of functional proteins at single CTC resolution has not yet been achieved, owing to the limited purity of the isolated CTC populations and a lack of single-cell proteomic approaches to handle and analyze rare CTCs. Her...
متن کاملMicrofluidic Device for Continuous Dielectrophoretic Separation of Cells in Division
This paper reports on a novel method for on-chip continuous separation of dividing and non-dividing cells based on differences in their dielectric properties. By means of two opposite dielectrophoretic force fields at multiple frequencies, the two populations of cells flowing through the microfluidic device are focused towards distinct equilibrium positions, which can be correlated to their cel...
متن کاملContinuous flow separation of particles within an asymmetric microfluidic device.
A microfluidic based device has been developed for the continuous separation of polymer microspheres, taking advantage of the flow characteristics of systems. The chip consists of an asymmetric cavity with variable channel width which enables continuous amplification of the particle separation for different size particles within the laminar flow profile. The process has been examined by varying...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RSC Advances
سال: 2019
ISSN: 2046-2069
DOI: 10.1039/c9ra08285a