Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo

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Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo

Drosophila embryogenesis is initiated by a series of syncytial mitotic divisions. The first nine of these divisions are internal, and are accompanied by two temporally distinct nuclear movements that lead to the formation of a syncytial blastoderm with a uniform monolayer of cortical nuclei. The first of these movements, which we term axial expansion, occurs during division cycles 4-6 and distr...

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Luna, a Drosophila KLF6/KLF7, Is Maternally Required for Synchronized Nuclear and Centrosome Cycles in the Preblastoderm Embryo

Krüppel like factors (KLFs) are conserved transcription factors that have been implicated in many developmental processes including differentiation, organ patterning, or regulation of stem cell pluripotency. We report the generation and analysis of loss-of-function mutants of Drosophila Klf6/7, the luna gene. We demonstrate that luna mutants are associated with very early embryonic defects prio...

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Cytoplasmic organization, nuclear migration, and nuclear division in the early syncytial Drosophila embryo

The generation of order and asymmetry at the cytoplasmic level is fundamental to many basic cellular functions including mitosis, directed cell movement, and developmental processes such as axis specification. All of these events are largely dependent on the cytoskeleton, which is responsible for organizing the cytoplasm of eukaryotic cells. The problem of cytoplasmic organization is particular...

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Aster migration determines the length scale of nuclear separation in the Drosophila syncytial embryo

In the early embryo of many species, comparatively small spindles are positioned near the cell center for subsequent cytokinesis. In most insects, however, rapid nuclear divisions occur in the absence of cytokinesis, and nuclei distribute rapidly throughout the large syncytial embryo. Even distribution and anchoring of nuclei at the embryo cortex are crucial for cellularization of the blastoder...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1993

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.122.1.113