Dual Targeting to Mitochondria and Chloroplasts: Characterization of Thr–tRNA Synthetase Targeting Peptide
نویسندگان
چکیده
منابع مشابه
Dual Targeting of Glutathione Reductase to Mitochondria and Chloroplasts
..................................................................................................................................... 4 List of Publications for the thesis............................................................................................. 5 Abbreviations .......................................................................................................................
متن کاملDual targeting and function of a protease in mitochondria and chloroplasts.
Here we show, using the green fluorescent protein (GFP) fusion system, that an Arabidopsis thaliana zinc-metalloprotease (AtZn-MP) is targeted to both mitochondria and chloroplasts. A deletion mutant lacking the amino-terminal 28 residues, with translation initiation at the second methionine residue, was imported into chloroplasts only. However, a mutated form of the full-length targeting pepti...
متن کاملA yeast mitochondrial leader peptide functions in vivo as a dual targeting signal for both chloroplasts and mitochondria.
A fusion protein was expressed in transgenic tobacco and yeast cells to examine the functional conservation of mechanisms for importing precursor proteins from the cytosol into mitochondria and chloroplasts. The test protein consisted of the mitochondrial leader peptide from the yeast precursor to cytochrome oxidase subunit Va (prC5) fused to the reporter protein chloramphenicol acetyltransfera...
متن کاملDual targeting of yeast catalase A to peroxisomes and mitochondria.
Yeast catalase A (Cta1p) contains two peroxisomal targeting signals (SSNSKF) localized at its C-terminus and within the N-terminal third of the protein, which both can target foreign proteins to peroxisomes. In the present study we demonstrated that Cta1p can also enter mitochondria, although the enzyme lacks a classical mitochondrial import sequence. Cta1p co-targeting was studied in a catalas...
متن کاملArabidopsis thaliana GLN2-encoded glutamine synthetase is dual targeted to leaf mitochondria and chloroplasts.
In higher plants, photorespiratory Gly oxidation in leaf mitochondria yields ammonium in large amounts. Mitochondrial ammonium must somehow be recovered as glutamate in chloroplasts. As the first step in that recovery, we report glutamine synthetase (GS) activity in highly purified Arabidopsis thaliana mitochondria isolated from light-adapted leaf tissue. Leaf mitochondrial GS activity is furth...
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ژورنال
عنوان ژورنال: Molecular Plant
سال: 2009
ISSN: 1674-2052
DOI: 10.1093/mp/ssp048