Dual species dynamic transcripts reveal the interaction mechanisms between Chrysanthemum morifolium and Alternaria alternata

Abstract Background Chrysanthemum ( morifolium ) black spot disease caused by Alternaria alternata is one of the plant’s most destructive diseases. Dual RNA-seq was performed to simultaneously assess their transcriptomes analyze potential interaction mechanism between two species, i.e., host and pathogen. Results C. A. were subjected dual at 1, 12, 24 h after inoculation, differential expression genes (DEGs) in both species identified. This analysis confirmed 153,532 DEGs chrysanthemum 14,932 , which involved plant-fungal interactions phytohormone signaling. Fungal such as toxin synthesis related enzyme cell wall degrading played important roles during infection. Moreover, a series key highly correlated with early, middle, or late infection stage identified, together regulatory network annotated Plant Resistance Genes database (PRGdb) Pathogen-Host Interactions (PHI-base). Highly identified stage, expanding our understanding interplay . Additionally, six each from selected for quantitative real-time PCR (qRT-PCR) assays validate output. Conclusions Collectively, data obtained this study enriches resources available research into that exist thereby providing theoretical basis development new cultivars resistance