Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting

Abstract Droplet microfluidics is a powerful tool for diverse range of biomedical and industrial applications such as single-cell biology, synthetic digital PCR, biosafety monitoring, drug screening, food, feed, cosmetic industries. As an integral part droplet microfluidics, on-chip multiplexed sorting has recently gained enthusiasm, since it enables real-time single droplets containing cells with different phenotypes into multiple bins. However, conventional methods are limited in throughput scalability. Here, we present high-throughput, scalable, by employing pair sequentially addressable dielectrophoretic arrays (SADAs) across microchannel on microfluidic chip. A SADA array electrodes, each which activated deactivated synchronization to the position speed flowing interest. The dual-SADA (dSADA) structure high-throughput deflection directions well-controlled manner. For proof-of-concept demonstration characterization dSADA, performed fluorescence-activated (FADS) 3-way dSADA at high 2450 droplets/s. Furthermore, show scalability also FADS 5-way 473