Dual Molecule Assay to Investigate the Mechanics of Homology Search and Recognition in Recombination
نویسندگان
چکیده
منابع مشابه
Mechanism of homology recognition in DNA recombination from dual-molecule experiments.
In E. coli homologous recombination, a filament of RecA protein formed on DNA searches and pairs a homologous sequence within a second DNA molecule with remarkable speed and fidelity. Here, we directly probe the strength of the two-molecule interactions involved in homology search and recognition using dual-molecule manipulation, combining magnetic and optical tweezers. We find that the filamen...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.266