Drosophilaengrailed-1, 10-phenanthroline chimeras as probes of homeodomain-DNA complexes
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چکیده
منابع مشابه
Engineering of DNA binding proteins into site-specific cutters: reactivity of Trp repressor-1,10-phenanthroline chimeras.
Trp repressor (TrpR) can be converted into a site-specific nuclease by chemical modification of the cysteine mutants TrpR D46C or TrpR E49C with 5-iodoacetamido-1,10-phenanthroline (OP). In the presence of cupric ion and 3-mercaptopropionic acid, TrpR-regulated operators are cleaved. The properties of these semisynthetic scission reagents have been compared. The E49C construct cleaves efficient...
متن کاملDNA as a catalyst and catalytic template for the racemisation of metal tris-phenanthroline complexes
[Fe(1,10-phenanthroline)3] 2+ is known to racemise in solution and DNA is known to shift the equilibrium point of the /-[Fe(1,10-phenanthroline)3] 2+ mixture from 50:50 to a position favouring a slight excess of the enantiomer. In this paper it is shown that DNA catalyses the racemisation reaction, presumably by providing vibrational energy to species that bind in a less favoured mode for t...
متن کاملStability and spectral properties of europium and zinc phenanthroline complexes as luminescent probes in high content cell-imaging analysis
a Medicinal Chemistry, Institute of Molecular Medicine, Trinity Centre for Health Sciences, Trinity College Dublin, St James's Hospital, Dublin 8, Ireland b School of Chemistry, SFI Tetrapyrrole Laboratory, Trinity College Dublin, Dublin 2, Ireland c Clinical Medicine, Institute of Molecular Medicine, Trinity Centre for Health Sciences, Trinity College Dublin, St James's Hospital, Dublin 8, Ire...
متن کاملLEWIS-BASE ADDUCTS OF LEAD(II) COMPOUNDS VI.* SYNTHESIS AND STRUCTURAL CHARACTERIZATION OF MONONUCLEAR (DIACETATO-0,O ')(1, 10-PHENANTHROLINE)
The synthesis and single-crystal X-ray structure determination of the title compound (phen)Pb(02CMe) is described. The complex crystallizes in the monoclinic space group P2 /c, a = 7.823(3), b = 12.421(4), c = 9.262(2) ?, ? = 79.92(3)", Z = 4, yielding R 0.048 for 3210 independent reflections. The coordination number around the lead atom is six. [Pb-N 2.54(2), 2.56(4)],[Pb-0, 2.19-2.22]
متن کامل2,9-Dimethyl-1,10-phenanthroline as Ligand in the Holo- and Hemidirected 1:1 and 1:2 Lead(II) Complexes
1:1 and 1:2 lead(II) complexes with 2,9-dimethyl-1,10-phenanthroline (Dmphen), {[Pb(Dmphen)(NO3)n] and [Pb(Dmphen)2(ClO4)2]} have been synthesized and characterized by CHN elemental analysis, IR, 1H NMR, 13C NMR and 207Pb NMR spectroscopy. The structure of these complexes was confirmed by X-ray crystallography. The single crystal X-ray data of [Pb(Dmphen)(NO3)n] show the complex to be polymeric...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1995
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.5560041105