Double Synergy Differential Test for detection of plasmid-mediated AmpC-type β-lactamases in Proteus mirabilis

Evaluation of a double synergy differential test (DSDT) for differential detection of ESBL and AmpC-type β-lactamases in Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis.

This work describes a simple and practical double synergy differential test (DSDT) that couples the detection of ESBLs and AmpC-type enzymes by means of a combo-disk approach using cefotaxime and ceftazidime as indicator substrates, and clavulanate and boronic acid as enzyme inhibitors. The DSDT was tested with a collection of 118 Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis st...

Plasmid-mediated carbencillin hydrolyzing beta-lactamases of Proteus mirabilis.

Since ABRAHAM and CHAIN') reported the production of beta((l)-lactamase by Escherichia coli, penicillinases have been shown to play an important role in the resistance of organisms against (3lactam antibiotics2.3). In many cases, the 46lactamases of Gram-negative bacteria have been shown to be mediated by R plasmids'), in contrast to chromosomally encoded drug resistance'). 46-Lactamases of Pro...

Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals

The incidence of resistance by Enterobacteriaceae to β-lactam/β-lactamase inhibitors combination is increasing in Egypt. Three phenotypic techniques, comprising AmpC disk diffusion and inhibition dependent methods using phenylboronic acid (PBA) and cloxacillin, were compared to PCR based method for detection of plasmid mediated AmpC β-lactamase in common urinary tract isolates. A total of 143 i...

PCR Detection of Plasmid Mediated TEM, SHV and AmpC β-Lactamases in Community and Nosocomial Urinary Isolates of Escherichia coli

Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli

Escherichia coli (E. coli) is a common opportunistic pathogen for nosocomial infection. The aim of the study was to examine the phenotype, genotype and epidemiology of plasmid-mediated AmpC β-lactamases in E. coli. In total, 96 clinical isolates of repeated E. coli were collected from different hospitals between August and October 2012. Using a cefoxitin disk diffusion method to identify the ph...