Double Prenylation by RabGGTase Can Proceed without Dissociation of the Mono-prenylated Intermediate
نویسندگان
چکیده
منابع مشابه
Double prenylation by RabGGTase can proceed without dissociation of the mono-prenylated intermediate.
Rab geranylgeranyltransferase (RabGGTase) catalyzes the prenylation of Rab proteins. Despite possessing a single active site, RabGGTase is able to add geranylgeranyl moieties onto each of the two C-terminal cysteine residues of Rab. We have studied the kinetics of Rab double prenylation employing a combination of a novel high pressure liquid chromatography (HPLC)-based in vitro prenylation assa...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m106470200