Double Labeling for Whole-Mount In Situ Hybridization in Mouse

Double labeling for whole-mount in situ hybridization in mouse.

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Double labeling with fluorescence in situ hybridization in Drosophila whole-mount embryos.

Previously, we described a technique for fluorescence in situ hybridization (FISH) in whole-mount Drosophila embryos using fluoresceinlabeled RNA probes (2). This technique provides several advantages over conventional alkaline phosphatasebased detection methods. For example, a fluorescent label is nondiffusible and so allows higher-resolution imaging. Furthermore, it allows the use of confocal...

Combined fluorescence in situ hybridization and immunocytochemistry double labeling in whole-mount Manduca sexta tissue.

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. The protocol is a modified version of the standard in situ hybridization usi...

Whole-mount in situ hybridization to mouse embryos.

The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sen...