Double immunoenzymatic staining by parallel incubation using monoclonal antibodies of different isotypes.
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چکیده
منابع مشابه
Double staining of ginsenosides by Western blotting using anti-ginsenoside Rb1 and Rg1 monoclonal antibodies.
Ginsenosides separated by silica gel TLC blotted to a polyvinylidene difluoride (PVDF) membrane was treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with anti-ginsenoside Rg1 and Rb1 monoclonal antibodies (MAbs). The newly established double staining with the Western blotting methods...
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Simultaneous detection of two antigens by immunostaining usually requires primary antibodies from two different species or a hapten modification of one of the antibodies if they are from the same species. A novel double staining method is described for immunodetection of two independent antigens using two mouse monoclonal antibodies. The principle of the method is the following: The first antig...
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ژورنال
عنوان ژورنال: The Journal of the Japanese Society of Clinical Cytology
سال: 1994
ISSN: 1882-7233,0387-1193
DOI: 10.5795/jjscc.33.93