Dose‐dependent density change of lens epithelial cells after in vivo UVR exposure
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چکیده
منابع مشابه
Lens growth and protein density in the rat lens after in vivo exposure to ultraviolet radiation.
PURPOSE To investigate lens growth after different doses of ultraviolet radiation (UVR) and to investigate the long-term effect of a near-threshold UVR dose on the refractive index distribution in the lens. METHODS Sprague-Dawley rats received UVR (lambda(MAX) = 300 nm) unilaterally during a 15-minute period. The exposure dose ranged from 0.1 to 20 kJ/m(2), and the rats were kept for up to 32...
متن کاملLens lactate dehydrogenase inactivation after UV-B irradiation: an in vivo measure of UVR-B penetration.
PURPOSE To elucidate the spatial distribution of inactivation of lactate dehydrogenase (LDH) in ultraviolet-B radiation (UVR-B)-exposed eyes. To determine the in vivo penetration depth of UVR-B in the lens. METHODS LDH activity in cornea and lens was investigated with an enzyme histochemical technique. Thirty rats were exposed in vivo to UVR-B of approximately 300 nm, and the eyes were enucle...
متن کاملCell Adhesion Molecule Expression in Human Lens Epithelial Cells After Corticosteroid Exposure
AIM The aim of the study was to investigate changes in cell adhesion molecule expression in human lens epithelial cells (HLEC) subjected to glucocorticoids. METHODS Human lens epithelial cells were exposed to different concentrations of dexamethasone for 24 hours. Cell adhesion molecule expression was studied by western blot and immunohistochemistry of vimentin, N-cadherin, E-cadherin, α-cate...
متن کاملBiological Response in Various Compartments of the Rat Lens after In Vivo Exposure to UVR-B Analyzed by HR-MAS H NMR Spectroscopy
METHODS. One eye in each of 28 6-week-old female albino Sprague-Dawley rats was exposed to in vivo 7.5 kJ/m UVR-B for 15 minutes. The contralateral eye was left unexposed. One week after irradiation, all rats were killed, and both lenses were isolated. Each lens was cored by a trephine, and the cylinder was sliced into three portions (anterior, nuclear, and posterior). The lens material that re...
متن کاملBiological response in various compartments of the rat lens after in vivo exposure to UVR-B analyzed by HR-MAS 1H NMR spectroscopy.
PURPOSE The purpose of the present study was to investigate metabolic changes in different compartments of the rat lens (anterior, nuclear, posterior, and equatorial) after exposure to an acute double threshold dose of ultraviolet-B radiation (UVR-B) by using high-resolution magic angle spinning (HR-MAS) (1)H nuclear magnetic resonance (NMR) spectroscopy and pattern recognition (PR) METHODS: me...
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ژورنال
عنوان ژورنال: Acta Ophthalmologica
سال: 2019
ISSN: 1755-375X,1755-3768
DOI: 10.1111/j.1755-3768.2019.5127