Donor cornea transfer from Optisol GS to organ culture storage: a two‐step procedure to increase donor tissue lifespan
نویسندگان
چکیده
منابع مشابه
Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan
PURPOSE Storage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC. METHODS Morphology was monitored by light and electron microscopy, expression of phenotypic and genotypic markers by immunohistochemistry and RT-PCR and chang...
متن کاملcomparison of donor cornea storage in optisol-gs and the central eye bank of iran (cebi) medium
purpose: optisol-gs is still the most popular cold storage medium used in the united states for short term preservation of donated corneas. cebi medium is a new cold storage medium made by the central eye bank of iran for donor cornea storage. this study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in optisol-gs versus in the cebi medium. m...
متن کاملEffects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.
PURPOSE A previous report has described the use of eye bank storage of cultured human limbal epithelial cells (HLECs) to provide a reliable source of tissue for treating limbal stem cell deficiency. In the present study, conventional organ culture (OC) storage and Optisol-GS (Bausch & Lomb, Irvine, CA) storage of cultured HLECs were compared. METHODS Three-week HLEC cultures were either organ...
متن کاملIs microbiological analysis of donor cornea transport culture media necessary?
PURPOSE To investigate microbial contamination of the transport medium. Examination of corneoscleral rims is not included in this series. METHODS Transport media of 63 consecutive grafts done at Tennent Institute of Ophthalmology, Glasgow, were collected for microbial examination. RESULTS None of the culture plates showed any growth after prolonged culture, and microscopy was negative in 10...
متن کاملStrategies to increase limited donor resources.
A number of strategies have been advocated to increase the number of lung donors including: 1) improvement in donor resuscitation; 2) better methods of lung preservation; 3) extension of the lung donor selection criteria; 4) development of a living-related lung donor programme; and 5) utilisation of nonheart beating donors. Other strategies such as the split-lung technique and techniques of lun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Ophthalmologica
سال: 2012
ISSN: 1755-375X,1755-3768
DOI: 10.1111/j.1755-3768.2012.02390.x