Donnan Membrane Potential

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Membrane Potentials in the Donnan Equilibrium

(The diagram gives, however, no indication of the hydrostatic pressure due to the liquid in the manometer tube, which is a necessary condition for equilibrium. For further details of the experiments Loeb's original papers and book s may be consulted.) AH and BH represent hydrogen electrodes; AK and BK, calomel electrodes connected to the solutions by saturated KC1. Loeb measured the potential d...

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Membrane Potentials in the Donnan Equilibrium. Ii

Measurements were made of electromotive force in the Donnan equilibrium of systems containing dilute solutions of protein and acid. Removal of the membrane produced a decrease of no more than 2 to 4 mv. in electromotive force, while the membrane potentials, as estimated by the usual arbitrary assumption, were of the order of 12 to 34 mv. Ion ratios, as calculated from analyses for total chlorid...

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Fixed charge and transport numbers in isolated pepper fruit cuticles from membrane potential measurements: Donnan and diffusion potential contributions

Membrane potential of native isolated pepper cuticles were measured with NaCl and CaCl2 solutions for a wide range of concentrations (10BC(M)B10). From membrane potential results the fixed charge concentration, X, and the transport number of the ions in the cuticular membrane were obtained. Taking into account the X value, an estimation of the Donnan potential contribution to the membrane poten...

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The relation between dicarbocyanine dye fluorescence and the membrane potential of human red blood cells set at varying Donnan equilibria

The fluorescence, F, of two dicarbocyanine dyes, diS-C3(5) and diI-C3(5), depends both on the membrane potential, E, and on the intracellular pH, pHc, or human red blood cells. Compositions of isotonic media have been devised in which the equilibrium Donnan potential, E, varies at constant pHc and in which pHc varies at constant E. Dye fluorescence measurements in these suspensions yield calibr...

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Donnan potential of rabbit skeletal muscle myofibrils I: electrofluorochromometric detection of potential.

The fluorescence of the dye CC-6 [(3-hexyl-2-(3-hexyl-2-benzoxazolinylidene)-1-propenyl)-benzoxazolium iodide] has been shown to indicate Donnan potentials in rabbit skeletal muscle myofibrils. These results are in agreement with previously published work in which the potentials were measured with microelectrodes on glycerol-extraced muscle fibers. The magnitude of the Donnan potential of the m...

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ژورنال

عنوان ژورنال: Nature

سال: 1944

ISSN: 0028-0836,1476-4687

DOI: 10.1038/153593b0