Does formylation of initiator tRNA act as a regulatory signal inE. coli?

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منابع مشابه

The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show...

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Mutants of Escherichia coli formylmethionine tRNA: a single base change enables initiator tRNA to act as an elongator in vitro.

We show that the absence of a Watson-Crick base pair at the end of the amino acid acceptor stem, which is a hallmark of all prokaryotic initiator tRNAs, is one of the key features that prevents them from acting as an elongator in protein synthesis. We generated mutants of Escherichia coli formylmethionine tRNA that have a base pair at the end of the acceptor stem. The mutants generated were C1-...

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How many initiator tRNA genes does Escherichia coli need?

Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell appears to be unaffected by the removal of one copy. However, the fitness of ...

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Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA.

Protein synthesis in eukaryotic organelles such as mitochondria and chloroplasts is widely believed to require a formylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation. Here we show that initiation of protein synthesis in yeast mitochondria can occur without formylation of the initiator methionyl-tRNA (Met-tRNA(fMet)). The formylation reaction is catalyzed by methionyl-tRNA formyl...

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Dependence of Mitochondrial Protein Synthesis Initiation on Formylation of the Initiator Methionyl-tRNA,

The effect of N’“-formyl-H,folate on mitochondrial peptide chain initiation has been studied in isolated mitochondria of Saccharomyces cerevisiae. The addition of N”‘-formyl-H,folate strongly stimulates the incorporation of amino acids into mitochondrial protein at both 6 and 15 mu Mg2+. Still higher stimulation (up to IO-fold) has been obtained in the production of de nouo synthesized initial ...

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1973

ISSN: 0014-5793

DOI: 10.1016/0014-5793(73)80823-3