Does Dry or Fresh Bee Bread Contain Clinically Significant, and Antimicrobial Agents Resistant Microorganisms?

Bee pollen, which is filled into the cells of honeycomb by honeybees to use in nutrition, enriched with digestive enzymes and organic acids from honey salivary gland secretions honeybees. Lactic acid fermentation takes place comb cell under anaerobic conditions bee bread formed. We have limited knowledge about bacteria yeasts are involved process. Samples collected 8 different beehives same apiary samples that were dried for 3 months stored deep freezer analyzed microbiologically. The 34 strains isolated fresh perga samples. No microorganism was growth identified conventional MALDI-TOF MS methods. as Aspergillus spp. (12), Rhizopus oryzae (6), Mucor circinelloides (1), Bipolaris (2), Trichoderma (3), Paecilomyces variotii Penicillium chrysogenum Kodamaea ohmeri Bacillus altitudinis/pumilus licheniformis B. megaterium Micrococcus luteus (1) Serratia marcescens (1). MICs values itraconazole, voriconazole, anidulafungin caspofungin had high (≥32 μg/ml) except amphotericinB, posaconazole. drugs (n:9/12) low (1 μg/ml). MIC (≤0.50 other itraconazole. P.chrysogenum found value (≤0.25 posaconazole, anidulafungin, caspofungin. Bipolaris, P.variotii K.ohmeri all antifungals. 
 determination microbiological load soon they hive allows us discuss beneficial harmful aspects these microorganisms, while absence microbial appropriate striking.