Does Bilirubin Cause Interference in Roche Creatinine Methods?
نویسندگان
چکیده
منابع مشابه
Bilirubin interference in kinetic creatinine determination.
194 CLINICAL CHEMISTRY, Vol. 25, No. 1, 1979 gas chromatography before and after acid (6 mol/L HC1) and alkaline (NaOH) hydrolysis. We frequently observe a peak in the lactic acid position after alkaline hydrolysis. After acid hydrolysis of the same urine no lactate isobserved, but a peak appears in the position of levulinic acid. The mass spectra of these two peaks were identical with those of...
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Bilirubin causes underestimation of serum creatinine in the Jaffé alkaline picrate assay. We report an approach for preventing bilirubin interference by pretreating serum samples with peroxidase and H2O2. The dissociation of bilirubin from albumin and its subsequent oxidation markedly reduces the bilirubin interference and enables accurate determination of creatinine concentrations by the Jaffé...
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A colorimetric peroxidase-coupled procedure for determination of creatinine in human serum and urine is described. A 30-s sample pre-treatment with bilirubin oxidase eliminates interference from endogenous bilirubin. The 4-aminoantipyrine-2-hydroxy-3,5-dichlorobenzenesulfonate chromogen system of this method is about fourfold more sensitive than current procedures that involve monitoring NAD(P)...
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The negative interference by bilirubin in the measurement of creatinine by the Jaffé procedure is well documented. We describe a procedure for the elimination of interfering bilirubin by oxidation to biliverdin through the addition of potassium ferricyanide before the alkaline picrate reagent. We detected no negative interference with the addition of as much as 600 mumol/L bilirubin. The proced...
متن کاملA solution to the problem of bilirubin interference with the kinetic Jaffé method for serum creatinine.
We measured bilirubin interference with the kinetic Jaffé method for serum creatinine. Both pooled sera with added bilirubin and icteric patients' sera were used and results with of which gave more nearly "true" values.
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2007
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2006.075846