DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha)

DNA ( cytosine - 5 ) - methyltransferase 3 B - [ Isoform 1 ]

A DNMT3B (DNMT3B , DNA (cytos ine-5)methyltransferase 3B; Swiss-Prot accession number: Q9UBC3) gene deletion construct in which exons 16–19 were deleted was used to generate Dnmt3b-null ES cells and animals. The deleted region encodes the highly conserved PC (proline cysteine) and ENV (glutamic acid, asparagine, valine) motifs that are crucial for catalytic activity [1,2]. Targeted replacement ...

DNMT 1 ( DNA ( cytosine - 5 - ) - methyltransferase 1 ) )

DNA ( cytosine - 5 ) - methyltransferase 1 - [ Isoform 1 ]

The murine equivalent of the human DNMT1 (DNA [cytosine-5]-methyltransferase 1; Swiss-Prot accession number: P26358) gene was deleted in ES cells via gene targeting [1]. Dnmt1-/cells possessed dramatically decreased genomic methylation and were viable; however, the mutation caused a homozygous lethal phenotype when introduced into the germline (see Table of experimental models for DNMT1) [1]. C...

Protein-facilitated base flipping in DNA by cytosine-5-methyltransferase.

DNA methylation, various DNA repair mechanisms, and possibly early events in the opening of DNA as required for transcription and replication are initiated by flipping of a DNA base out of the DNA double helix. The energetics and structural mechanism of base flipping in the presence of the DNA-processing enzyme, cytosine 5-methyltransferase from HhaI (M.HhaI), were obtained through molecular dy...

Mammalian DNA cytosine-5 methyltransferase interacts with p23 protein.

In higher eukaryotic genomes, methylated cytosine residues (m(5)C) are distributed in heritable, cell-type-specific patterns, which are believed to be involved in the control of gene expression, developmental regulation and genomic imprinting. These methylation patterns are established and maintained by DNA cytosine-5 methyltransferase (MTase), a approximately 1500 amino acid enzyme containing ...