DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening
نویسندگان
چکیده
منابع مشابه
DNA transformation of Leishmania infantum axenic amastigotes and their use in drug screening.
Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA electroporation of Leishmania axenic amastigotes were systematically studied using luciferase-mediat...
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Objective(s): Leishmaniasis is endemic in 88 countries. Amastigote forms of Leishmania are experts at exploiting host cell processes to establish infection. Monoclonal antibodies are key reagents used in the diagnosis of infectious and non-infectious diseases. The aim of this study was to produce monoclonal antibodies against axenic amastigotes of the Leishmania infantum strain in Iran.Material...
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Experimental infections with visceral Leishmania spp. are frequently performed referring to stationary parasite cultures that are comprised of a mixture of metacyclic and non-metacyclic parasites often with little regard to time of culture and metacyclic purification. This may lead to misleading or irreproducible experimental data. It is known that the maintenance of Leishmania spp. in vitro re...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2001
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.45.4.1168-1173.2001