منابع مشابه
In silico Simulations for DNA Shuffling Experiments
DNA shuffling is a powerful method used for in vitro evolute molecules with specific functions and has application in areas such as, for example, pharmaceutical, medical and agricultural research. The success of such experiments is dependent on a variety of parameters and conditions that, sometimes, can not be properly pre-established. Here, two computational models predicting DNA shuffling res...
متن کاملPredicting crossover generation in DNA shuffling.
We introduce a quantitative framework for assessing the generation of crossovers in DNA shuffling experiments. The approach uses free energy calculations and complete sequence information to model the annealing process. Statistics obtained for the annealing events then are combined with a reassembly algorithm to infer crossover allocation in the reassembled sequences. The fraction of reassemble...
متن کاملComputational and experimental analysis of DNA shuffling.
We describe a computational model of DNA shuffling based on the thermodynamics and kinetics of this process. The model independently tracks a representative ensemble of DNA molecules and records their states at every stage of a shuffling reaction. These data can subsequently be analyzed to yield information on any relevant metric, including reassembly efficiency, crossover number, type and dist...
متن کاملRandom DNA fragmentation with endonuclease V: application to DNA shuffling.
The enzyme endonuclease V nicks uracil-containing DNA at the second or third phosphodiester bond 3' to uracil sites. I applied the enzyme to random fragmentation of DNA to revise the complex DNA shuffling protocol. The merit of using endonuclease V is that cleavage occurs at random sites and the length of the fragments can easily be adjusted by varying the concentration of dUTP in the polymeras...
متن کاملFamily shuffling with single-stranded DNA.
1. Introduction Family shuffling, which generates chimeric progeny genes by recombining a set of naturally occurring homologous genes, is an extremely powerful approach for in vitro protein evolution. In comparison with other in vitro protein evolution methods, family shuffling has the advantage of sampling a larger portion of the sequence space that has been proven functionally rich by nature....
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 1996
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt0396-366