DNA Polymerase Activity Associated with RNA Tumor Viruses
نویسندگان
چکیده
منابع مشابه
DNA polymerase activity associated with RNA tumor viruses.
C-type RNA viruses, originating in mouse, cat, hamster, and viper, catalyze the synthesis of DNA from its constituent deoxyribonucleoside triphosphates. Both the rate and extent of the reaction were significantly enhanced by brief treatment of the intact virus with ether. A proportion of the newly synthesized DNA was associated with virions when intact virus was used as template; this was not t...
متن کاملRNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.
The role of ribonucleic acid (RNA) in deoxyribonucleic acid (DNA) synthesis with the purified DNA polymerase from the avian myeloblastosis virus has been studied. The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg(2+), and a variety of RNA templates including those isolated from avian myeloblastosis, Rous sarcoma, and Rauscher leukemia viruse...
متن کاملAffinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.
A solid phase immunoadsorbent specific for RNA-dependent DNA polymerase from murine and feline RNA tumor viruses has been prepared. The enzymes from murine and feline virus but not from avian virus bind to columns of this material. Bound enzymes can be eluted in active form. This method has permitted selective purification of viral enzyme from crude extracts of virus-transformed cells, since th...
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Columns of (dT)(12-18)-cellulose provide a one-step enrichment procedure for RNA-dependent DNA polymerase. The enzyme of the virus from RD-114 cells, as well as that from Rauscher murine leukemia virus, have been purified in this way. The preference of viral as compared to cellular DNA polymerases for (dT)(12-18) as a primer is reflected in the fact that the DNA polymerases of uninfected cells ...
متن کاملAntibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses.
Sera from rats bearing transplantable tumors induced by murine C-type tumor viruses contain an inhibitor of the activity of the viral RNA-dependent DNA polymerase. The inhibitor is shown to be an immunoglobulin (IgG) directed against the enzyme. Antiserum made in rabbits against partially purified murine leukemia virus polymerase also inhibits the polymerases of other mammalian C-type RNA-conta...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1970
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.67.1.143