DNA Nanomaterials for Delivery of Clustered Regularly Interspaced Short Palindromic Repeats/Cas Systems

The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein (CRISPR/Cas) system has been exploited as an efficient gene editing tool with precise site specificity and high efficiency; the delivery of CRISPR/Cas is critical for efficacy therapeutics remains challenging. DNA nanomaterials are widely utilized promising carriers agents because their excellent molecular recognition capabilities, sequence programmability, biocompatibility. Herein, recent advances in systems summarized. Based on construction strategy, nanomaterial-based categorized branched DNA-based nanostructures or rolling circle amplification (RCA)-based nanostructures. Representative studies design highlighted. current challenges opportunities development also discussed. It envisioned that nanotechnology, will open up new possibilities CRISPR/Cas-based therapeutics.