DNA circles with cruciforms from Isospora (Toxoplasma) gondii

Detection of genomic Toxoplasma gondii DNA and anti-Toxoplasma antibodies

Toxoplasmosis is a disease of zoonotic nature; being reported to be widespread in animals and humans. Serological diagnosis represents the first and the most widely used approach to define the stage of toxoplasmosis and diagnosis of primary and late infection in pregnancy can be improved by determination of Toxoplasma DNA. Eighty-eight and 88 coagulated and non coagulated blood samples were col...

Toxoplasma gondii

A total of 349 human serum samples were examined for anti-Toxoplasma antibodies by the Murex Single Use Diagnostic System (SUDS) qualitative screening test, indirect hemagglutination assay (IHA), and indirect immunofluorescence assay (IFA). Concordant results with SUDS and IHA were obtained for 91.9% of serum samples; 8.9% were SUDS+ and IHA-; none were SUDSand IHA+. Comparison of the SUDS assa...

Toxoplasma gondii

The performance of a direct agglutination test for the detection of toxoplasma specific IgG immunoglobulin was compared with that ofthe latex agglutination test. The direct agglutination test was less sensitive but more specific than the latex agglutination test. Quantitative results were not directly comparable, reflecting the different antibody profiles detected in each assay. The direct aggl...

Toxoplasma gondii serosurvey in Golden Jackals from Golestan province, Iran

Toxoplasma gondii as an apicomplexan zoonotic parasite is able to infect humans and almost all other warm-blooded animals. Wild and domestic felids are the main definitive hosts. Also, canids such as dogs and jackals have been introduced as mechanical vectors since T. gondii oocysts can pass their intestinal tract and spread by feces in environment. Toxoplasmosis is a common zoonotic disease in...

Purification of Toxoplasma gondii from host cells.