DNA barcodes and microsatellites: How they complement for species identification in the complex genus <i>Tamarix</i> (Tamaricaceae)

Abstract DNA barcoding allows the identification of an organism by comparing sequence selected regions (barcodes) with a previously compiled database, and it can be useful for taxonomic species in complex genera, such as Tamarix . Many this genus show convergent morphology, which leads to frequent errors their identification. Highly variable genetic markers, microsatellites or short repeats (SSR), could used differentiate where barcodes fail. Here, we tested ability both, 5 different marker ( rbcL , matK ITS, trnH ‐ psbA ycf1 ), 14 microsatellites, properly identify species, especially those from Mediterranean Basin, compared pros cons analytical methods certain The two‐locus + ITS were best‐performing combinations, allowing up 69% 70%, respectively, correct However, failed phylogenetically close groups, many species. use SSR aid combination both types data (DNA SSR) improved success. was relevant detecting presence hybridization processes, are common genus. caution must exercised when choosing clustering since lead very results.