Diversity of Omega Glutathione Transferases in mushroom-forming fungi revealed by phylogenetic, transcriptomic, biochemical and structural approaches

The Omega class of glutathione transferases (GSTs) forms a distinct within the cytosolic GST superfamily because most them possess catalytic cysteine residue. human 1 isoform was first characterized twenty years ago, but it took work to clarify roles isoforms. Concerning kingdom fungi, little is known about cellular functions (GSTOs), although they are widely represented in some these organisms. In this study, we re-assess phylogeny and classification GSTOs based on 240 genomes mushroom-forming fungi (Agaricomycetes). We observe that number not only extended order Polyporales also other orders such as Boletales. Our analysis leads new which fungal divided into two Types A B. residue Type-A either or serine, while Type-B cysteine. present study focuses Trametes versicolor GSTO isoforms Transcriptomic data show constitutive enzymes inducible ones. crystallographic reveals substantial structural differences between types have similar biochemical profiles tested conditions. Additionally, ability bind antioxidant molecules wood polyphenols possible binding sites observed from X-ray structures. multiplication could allow organisms adapt more easily environments.