Diversity for morphological traits, flowering time and leaf isoflavone content among ecotypes of

Trifolium subterraneum L. subsp. yanninicum is a pasture legume that widely grown in medium and high rainfall areas of southern Australia shows waterlogging tolerance. This study investigated diversity within corresponding to eco-geographic variables, which may help identify adapted parents with new traits for genetic improvement. Diversity 10 morphological traits, flowering time leaf isoflavone content was using 108 ecotypes derived from wild Mediterranean populations cultivars, as spaced plants. Among the ecotypes, range 94–149 days after sowing, contents formononetin, genistein biochanin A were 0.05–1.38%, 0.73–2.33% 0.15–2.10% dry matter, respectively. Leaf markings also varied considerably. size petiole length correlated at each growth stage. Later genotypes had larger leaves, longer petioles, internodes thicker stems flowering, but smaller leaves shorter petioles both 63 88 sowing. Contents unrelated, negatively associated formononetin. Flowering weak positive influence on A, negative All among (except stem diameter mark crescent size) significantly least one 22 variables their collection sites. Precipitation altitude more influential than temperature. The found sufficient broaden narrow base current cultivars; however, other agronomically important need be considered. Further result targeted collection, particularly not represented annual genebanks.