Dithiothreitol and the translocation of preprolactin across mammalian endoplasmic reticulum.
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چکیده
منابع مشابه
Dithiothreitol and the translocation of preprolactin across mammalian endoplasmic reticulum
The translocation mode of preprolactin (pPL) across mammalian endoplasmic reticulum was reinvestigated in light of recent findings that nascent secretory polypeptides synthesized in the presence of a highly reducing environment could be translocated posttranslationally and independently of their attachment to the ribosome (Maher, P. A., and S. J. Singer, 1986, Proc. Natl. Acad. Sci. USA, 83:900...
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Translocation-competent microsomal membrane vesicles of dog pancreas were shown to selectively bind nascent, in vitro assembled polysomes synthesizing secretory protein (bovine prolactin) but not those synthesizing cytoplasmic protein (alpha and beta chain of rabbit globin) . This selective polysome binding capacity was abolished when the microsomal vesicles were salt-extracted but was restored...
متن کاملTranslocation of proteins across the endoplasmic reticulum.
Translocation-competent microsomal membrane vesicles of dog pancreas were shown to selectively bind nascent, in vitro assembled polysomes synthesizing secretory protein (bovine prolactin) but not those synthesizing cytoplasmic protein (alpha and beta chain of rabbit globin) . This selective polysome binding capacity was abolished when the microsomal vesicles were salt-extracted but was restored...
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The rough endoplasmic reticulum is a major site of protein biosynthesis in all eukaryotic cells, serving as the entry point for the secretory pathway and as the initial integration site for the majority of cellular integral membrane proteins. The core components of the protein translocation machinery have been identified, and high-resolution structures of the targeting components and the transp...
متن کاملProtein Translocation Across the Endoplasmic Reticulum Membrane
Proteins to be secreted from eukaryotic cells are delivered to the extracellular space after trafficking through a secretory pathway composed of several complex intracellular compartments. Secretory proteins are first translocated from the cytosol into the endoplasmic reticulum (ER), after which they travel by vesicular trafficking via various intermediate destinations en route to the plasma me...
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ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1987
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.105.4.1555