Distribution of Yeast Cell Wall Lytic Activity among Microorganisms
نویسندگان
چکیده
منابع مشابه
Cell wall-lytic activity in (j&Awn&Wa /wsca
The soluble fraction of homogenates of synchronous CMorg#a /w^ca was tested for carbohydrate-lyzing activities. With isolated cell walls and /?-l,4-mannan or carboxymethyl cellulose as substrates, a sharp increase in activity occurred shortly before release of the daughter cells followed by a decline during release. The lytic activities were partially purified by ammonium sulphate precipitation...
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A cell-wall-degrading activity was solubilized from young cells and from mother cell walls of CMo/W/a /w^ca by treatment with L i C l . The cytoplasmic enzyme hexokinase was not detectable in these extracts. The LiCl-solubilized activity increased in the cell cycle parallel to the release of autospores. The enzyme was purified on a chromatofocusing column followed by gel filtration. Sodium dode...
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Proteins from intercellular fluid extracts of chemically stressed barley (Hordeum vulgare L.) leaves were separated by native polyacrylamide gel electrophoresis at alkaline or acid pH. Polyacrylamide gels contained Saccharomyces cerevisiae (bakers' yeast) or Schizosaccharomyces pombe (fission yeast) crude cell walls for assaying yeast wall lysis. In parallel, gels were overlaid with a suspensio...
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The autolytic release of fl-fructofuranosidase (EC 3.2.1.26) and acid phosphatase (EC 3.1.3.2) from the cell wall of yeast was studied under different organic solvents and with a variety of pII and temperature conditions. /3Fructofuranoeidase (but not acid phosphatase) was found to be a convenient marker for studying changes in the wall structure. The ethyl acetate-treated cell was characterize...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1972
ISSN: 0002-1369
DOI: 10.1080/00021369.1972.10860306