Distribution and redistribution of pancreatic islet cell surface antigen reactive with islet cell surface antibody in the rat
نویسندگان
چکیده
منابع مشابه
Restricted islet-cell reactive T cell repertoire of early pancreatic islet infiltrates in NOD mice.
The mechanisms responsible for initiating autoimmune diabetes remain obscure. Here, we describe a method for identifying both the alpha- and beta-chains of the T cell receptor (TCR) from individual pancreatic islet-infiltrating T cells at the earliest stages of disease in nonobese diabetic mice (NOD). Analysis of the TCR repertoire of these early islet infiltrates reveals enrichment for a small...
متن کاملProduction of anti-rat islet cell surface antibody in guinea pigs.
Anti-rat islet serum was prepared in guinea pigs by multiple subcutaneous inoculations of rat islets homogenates emulsified in complete Freund's adjuvant (CFA). The anti-rat islet serum was cytotoxic against rat spleen cells in the presence of complement and the nonspecific antibodies were observed with homogenates of rat livers and spleens. After absorption, the serum lost the cytotoxicity aga...
متن کاملAltered Pancreatic Islet Function and Morphology in Mice Lacking the Beta-Cell Surface Protein Neuroligin-2
Neuroligin-2 is a transmembrane, cell-surface protein originally identified as an inhibitory synapse-associated protein in the central nervous system. Neuroligin-2 is also present on the pancreatic beta-cell surface, and there it engages in transcellular interactions that drive functional maturation of the insulin secretory machinery; these are necessary for normal insulin secretion. The effect...
متن کاملCell-to-cell communication in rat pancreatic islet monolayer cultures is modulated by agents affecting islet-cell secretory activity.
Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with the fluorescent dye Lucifer Yellow CH and the cultures were observed by combined phase contrast and fluorescent microscopy. The dye spread from an injected cell directly into neighboring islet cells, and successive microinjections of dye into different cells defined territories comprised of 2-6 communicati...
متن کاملComparative immunocytochemistry of isolated rat & monkey pancreatic islet cell types.
BACKGROUND & OBJECTIVES Data on comparative distribution of the islet cell types in the Indian bonnet monkeys and rats are not available. The aim of the present study was to compare the arrangement of the three islet cell types in the native and in isolated rat and Indian bonnet monkey islets by immunocytochemistry. METHODS Rat islets isolated by chopped tissue collagenase digestion method an...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1983
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf00297393