Disinfection Efficacy of Tobamovirus-Contaminated Soil in Greenhouse-Grown Crops

The tobamoviruses tomato brown rugose fruit virus (ToBRFV) and cucumber green mottle mosaic (CGMMV) have caused severe crop damages worldwide. Soil-mediated dispersion of the mechanically transmitted constitute a major hindrance toward mitigating disease spread in crops carefully planted under sanitized conditions. Tobamoviruses are viable for months soil plant debris more than year adhere to clay. However, low percentage infectious foci occur following tobamovirus-infected growing cycle, rendering disinfection studies several contaminated plots inconclusive large-scale productions. We therefore formulated rigorous platform studying disinfectant efficacy greenhouses by pouring inoculum planting pits prior treatment truncating seedling roots before planting, which was otherwise conducted found that chlorine-based Taharan significantly efficient preventing ToBRFV CGMMV plants, respectively. KlorBack often as good Taharan. In addition, formulation chlorinated tri-sodium phosphate used at nonphytotoxic 3% concentration showed efficiency similar effect on infection only. Our study provided small-scale evaluation necessary application tobamovirus-contaminated soil, commonly occurs commercial greenhouses.