Discovery of Surfactant-Like Peptides from a Phage-Displayed Peptide Library
نویسندگان
چکیده
منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
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Enzyme inhibitory peptides with a loop structure stabilized using an antiparallel β-sheet scaffold (β-loop peptide) were obtained from a designed peptide phage library. Human dihydrofolate reductase (hDHFR) was used as the target enzyme. The obtained β-loop peptides were competitive inhibitors of hDHFR with micromolar inhibition constants and dissociation constants.
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Experimental section General All chemicals and solvents for peptide synthesis were of reagent or HPLC grade and were used without further purification. TARON resin was purchased from Clontech. Dihydrofolate (DHP), nicotinamide adenine dinucleotide phosphate (NADPH) and methotrexate (MTX) were purchased from Wako Pure Chemical Industries. Biotin-AC5-OSu and 2-morpholinoethanesulfonic acid monohy...
متن کاملIdentification of high-affinity VEGFR3-binding peptides through a phage-displayed random peptide library
OBJECTIVE Vascular endothelial growth factor (VEGF) interaction with its receptor, VEGFR-3/Flt-4, regulates lymphangiogenesis. VEGFR-3/Flt-4 expression in cancer cells has been correlated with clinical stage, lymph node metastasis, and lymphatic invasion. The objective of this study is to identify a VEGFR-3/Flt-4-interacting peptide that could be used to inhibit VEGFR-3 for ovarian cancer thera...
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ژورنال
عنوان ژورنال: Viruses
سال: 2020
ISSN: 1999-4915
DOI: 10.3390/v12121442