Discovery of anti-cell migration activity of an anti-HIV heterocyclic compound by identification of its binding protein hnRNP M

• An anti-HIV heterocyclic compound BMMP to induce abnormal core uncoating. hnRNP M as a BMMP-binding protein. Anti-cell migration activity of BMMP. Function suppress CD44 mRNA expression via regulation the M. One sometimes shows two biological functions, becoming important aspect recent drug discovery. This study began with an attempt confirm previously reported molecular mechanism anti-human immunodeficiency virus (HIV) [2-(benzothiazol-2-ylmethylthio)-4-methylpyrimidine], i.e., induction uncoating viral at post-entry step. Our mechanistic gave results consistent this mechanism. We further attempted find out target by pulldown approach using synthesized biotinylated (Biotin-BMMP) and successfully identified heterogenous nuclear ribonucleoprotein (hnRNP M) protein was found not be accountable for As has been promote cancer metastasis, we tested that suppressed human lung carcinoma cell line A549 stimulated transforming growth factor-β (TGF-β). Mechanistic showed Furthermore, six new derivatives were synthesized, patterns their activities against HIV-1 uniform, suggesting anti-cell are independent. Taken together, newly discovered identification its binding chemical biology approach.