Disc Gel Electrophoresis of Caseins Treated with Proteolytic and Glycolytic Enzymes

Gel Electrophoresis for Investigating Enzymes with Biotechnological Application

Serum lipoprotein estimation by polyacrylamide gel disc electrophoresis.

A simple, rapid method is described for separating the serum lipoproteins into clear, discrete and reproducible bands by polyacrylamide disc gel electrophoresis. The sample is prestained with Sudan Black B in a sample gel and resolved by electrophoresis in a discontinuous pH system consisting of a sample gel, concentrating gel and separating gel. 30 young healthy males and 20 young healthy fema...

Germination also causes decomposition of carbohydrates and proteins, when glycolytic and proteolytic enzymes are

Malting is usually carried out on intact barley seed, while hull is still attached to it. In this study, oat grain with and without hull was subjected to controlled germination to optimize its enzymes activity, in such a way that lipase has the lowest and αamylase and proteinase the highest activities. Since pH has a great impact on the activity of the enzymes, the pH of germination media was s...

Proteolytic enzymes.

Leipner, J. and R. Saller (2000). "Systemic enzyme therapy in oncology: effect and mode of action." Drugs 59(4): 769-80. Plant extracts with a high content of proteolytic enzymes have been used for a long time in traditional medicine. Besides proteolytic enzymes from plants, 'modern' enzyme therapy additionally includes pancreatic enzymes. The therapeutic use of proteolytic enzymes is partly ba...

High pressure effects on proteolytic and glycolytic enzymes involved in cheese manufacturing.

The activity of chymosin, plasmin, and Lactococcus lactis enzymes (cell envelope proteinase, intracellular peptidases, and glycolytic enzymes) were determined after 5-min exposures to pressures up to 800 MPa. Plasmin was unaffected by any pressure treatment. Chymosin activity was unaffected up to 400 MPa and decreased at 500 to 800 MPa. Fifty percent of control chymosin activity remained after ...