Directional and direct cloning strategy for high-throughput generation of recombinant baculoviruses
نویسندگان
چکیده
منابع مشابه
Directional and direct cloning strategy for high-throughput generation of recombinant baculoviruses.
The baculovirus expression vector system (BEVS) has become one of the most widely used systems for routine protein expression. We have developed an improved strategy to clone foreign genes directionally and directly into the baculovirus genome vector via a one-step procedure to generate recombinant viruses in a week. In this work, we constructed a host strain Escherichia coli DH10BacHB1.1, whic...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2006
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112258