Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides
نویسندگان
چکیده
منابع مشابه
Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides
Bacterial cell-surface display systems coupled with quantitative screening methods offer the potential to expand protein engineering capabilities. To more fully exploit this potential, a unique bacterial surface display scaffold was engineered to display peptides more efficiently from the surface exposed C- and N-termini of a circularly permuted outer membrane protein. Using directed evolution,...
متن کاملPuzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملFunctional Surface Display of Laccase in a Phenol-Inducible Bacterial Circuit for Bioremediation Purposes
Background: Phenolic compounds, which are produced routinely by industrial and urban activities, possess dangers to live organisms and environment. Laccases are oxidoreductase enzymes with the ability of remediating a wide variety of phenolic compounds to more benign molecules. The purpose of the present research is surface display of a laccase enzyme with adhesin involved in diffuse adhesion (...
متن کاملEnvironmental monitoring of display cases in museums to consider the impact of display cases characteristics on their inner air quality
While technical characteristics, such as material used in the construction of display cases and their dimensions are important factors in terms of presentation quality as well as attracting the attention of the visitors, they can influence the inner air quality of the display case and preservation conditions of the exhibits too. This study intends to examine the effect of the technical characte...
متن کاملformation and evolution of regional organizations: the case study of the economic cooperation organization (eco)
abstract because of the many geopolitical, geo economical and geo strategically potentials and communicational capabilities of eco region, members can expand the convergence and the integration in base of this organization that have important impact on members development and expanding peace in international and regional level. based on quality analyzing of library findings and experts interv...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2008
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/gzn020