Direct selection of recombinant plasmids with chlorate
نویسندگان
چکیده
منابع مشابه
Repressor titration: a novel system for selection and stable maintenance of recombinant plasmids.
The propagation of recombinant plasmids in bacterial hosts, particularly in Escherichia coli, is essential for the amplification and manipulation of cloned DNA and the production of recombinant proteins. The isolation of bacterial transformants and subsequent stable plasmid maintenance have traditionally been accomplished using plasmid-borne selectable marker genes. Here we describe a novel sys...
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Ligation of a DNA restriction fragment into a plasmid vector, followed by isolation of a recombinant plasmid, is a fundamental procedure in molecular biology. Standard protocols require at least two days to go from unligated DNA to the isolation of sufficient quantities of recombinant plasmid for restriction enzyme analysis or DNA sequencing (1,3). The two most timeconsuming steps involve the s...
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We have used chloroquine/agarose gel electrophoresis and a blot-hybridization technique to study the modulation of superhelicity of extrachromosomal DNA in mammalian cells. The high sensitivity of the procedure has allowed us to measure the change in the specific linking difference or superhelical density (sigma) of a plasmid, psvo alpha 1p3d, after its introduction into COS-7 cells by DNA tran...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1987
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1987.tb02123.x