Direct Detection and Sequencing of Damaged DNA Bases
نویسندگان
چکیده
منابع مشابه
DNA sequencing with direct transfer electrophoresis and nonradioactive detection.
which usually takes less than an hour. The database should be monitored for random distribution of sequences; accumulation of specific sequences, which indicates nonrandom sonication or some cloning problem, will become apparent early on. In practice, however, sonication seems to produce random libraries from DNAs varying widely in base composition and from a variety of organisms. Some minor ed...
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Contamination by present-day human and microbial DNA is one of the major hindrances for large-scale genomic studies using ancient biological material. We describe a new molecular method, U selection, which exploits one of the most distinctive features of ancient DNA--the presence of deoxyuracils--for selective enrichment of endogenous DNA against a complex background of contamination during DNA...
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The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce readable extension products greater than 1000 bases having uniform band intensities. A combinati...
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ژورنال
عنوان ژورنال: Genome Integrity
سال: 2011
ISSN: 2041-9414
DOI: 10.1186/2041-9414-2-10